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Thus, enrichment of peptides from digested samples using anti-peptides antibodies could alleviate these issues increasing the sensitivity of the targeted assay.

  • It is critical to bypass or account for this background signal in order to glean meaningful information about your target.

  • This technique monitors the amplification of the target in real-time via a target-specific fluorescent signal emitted during amplification.

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Competing interests The authors declared that no potential conflicts of interest with respect to authorship, the research, and the publication of this article do exist.

  • Open Access This article is licensed under a Creative Commons Attribution 4.

  • These multiple series of cycles indicate viruses in the body.




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