Choosing the right cuvette involves selecting the right material and the correct size based on your sample and instrumentation.
For example Naphthalene C 6H 6 absorbs at 268nm Anthracene absorbs at 311nm Tetracene absorbs at 476nm Effect of substitution of auxochrome Benzene is a less effective chromophore, the substitution of a polar group to it causes an increase in λ max in the visible region and hence ε max value increase.
A sample is placed behind the integrating sphere.