Patients were only evaluated by gender, not further characteristics, and by their Ct values no correlation with age is given, and Ct values for N- and RdRp gene amplification number, as shown in the supplementary Table.
Today, computer software calculates this exact point and all modern real-time cyclers have an automatic threshold line setting.
Of the 114 patients, 64 56.
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Master Mixes Fluorescence emission can be affected by pH and salt concentration in a solution.